Journal: Biomedicines

Article Title: Effects of Continuous Theta Burst Stimulation on Behavior and NMDA Receptor Subunits in the Trimethyltin-Induced Alzheimer’s-like Disease Model

doi: 10.3390/biomedicines14020391

Figure Lengend Snippet: Representative immunoblots and quantitative data of Western blot analysis of target proteins expression in hippocampal P2 fraction: ( A ) vGlut1, ( B ) NR1, ( C ) NR2A, ( D ) NR2B. Data were statistically analyzed by one-way ANOVA, followed by Tukey’s post hoc test, and expressed as a percentage of the mean values of the CONT group ± SEM (dots in the graphs represent values of individual animals). The control group is shown in the representative blot images above the graphs but was not included as a separate bar in the graphs to maintain clarity in the main comparisons. Symbols indicate significant differences compared to control: ** p < 0.01, *** p < 0.001, **** p < 0.0001, or Sham cTB. S group: ### p < 0.001, #### p < 0.0001, number of animals per group: 4–6. The can be downloaded at .

Article Snippet: NR1 , Cell Signaling Technology, Danvers, MA, USA, #5704 RRID: AB_1904067 , rabbit monoclonal; 1:1000.

Techniques: Western Blot, Expressing, Control

Journal: Exploration

Article Title: Long non‐coding RNA CASC15 enhances learning and memory in mice by promoting synaptic plasticity in hippocampal neurons

doi: 10.1002/EXP.20230154

Figure Lengend Snippet: Hippocampal CA1 neurons lost synaptic function behind 2610307P16Rik knockout. (A) Western blot was conducted to detect the expression levels of PSD95, SNAP25 and Synaptotagmin1. (B) Quantitative analysis of the expression levels of PSD95, SNAP25 and Synaptotagmin1, and data were normalized to the expression of 2610307p16Rik wild‐type group (mean ± SEM, n = 3). Student′s t ‐test * p < 0.05, ** p < 0.01. (C) Western blot was conducted to detect the expression levels of AMPA and NMDA receptor protein. (D) Quantitative analysis of the expression levels of NR2B, NR1, GluA2+3, GluA2, GluA1, and data were normalized to the expression of 2610307p16Rik wild‐type group (mean ± SEM, n = 3). Student′s t ‐test * p < 0.05, ** p < 0.01. (E) Representative traces of mEPSC in the CA1 region of hippocampal. (F,G) Quantification of mEPSC amplitude (mean ± SEM, n = 3). Student′s t ‐test ** p < 0.01. (H,I) Quantification of mEPSC frequency (mean ± SEM, n = 3). Student′s t ‐test * p < 0.05.

Article Snippet: Membranes were blocked in 5% non‐fat milk with phosphate buffered saline (PBS) Tween 20 (Cat#28352, ThermoFisher, USA), and the primary antibody (SNAP25 antibody, 1:400, Cat#3926, Cell Signaling Technology, USA); (Synaptotagmin‐1 Antibody, 1:400, Cat#3347, Cell Signaling Technology, USA); (PSD95 Antibody, 1:500, Cat#2507, Cell Signaling Technology, USA); (NR2B Antibody, 1:300, Cat#4207, Cell Signaling Technology, USA); (NR1 Antibody, 1:300, Cat# 5704, Cell Signaling Technology, USA); (GluA2+3 Antibody, 1:500, Cat#PA1‐4660, ThermoFisher, USA); (GluA2 Antibody, 1:500, Cat#13607, Cell Signaling Technology, USA); (GluA1 Antibody, 1:500, Cat#13185, Cell Signaling Technology, USA); (β‐Actin Antibody, 1:500, Cat#3700, Cell Signaling Technology, USA); (NTF3 Antibody, 1:300, Cat#PA1‐14861, ThermoFisher, USA); (FMRP Antibody, 1:500, Cat#4317, Cell Signaling Technology, USA) was added and co‐incubated at 4°C for 16 h. Corresponding secondary antibody (Goat anti‐Rabbit IgG (H+L) Secondary Antibody, HRP, 1:2000, Cat#31460, Cell Signaling Technology, USA); (Goat anti‐Mouse IgG (H+L) Secondary Antibody, HRP, 1:2000, Cat#31430, Cell Signaling Technology, USA) was introduced before bands visualization through Pierce ECL Western (Cat#32209, ThermoFisher, USA).

Techniques: Knock-Out, Western Blot, Expressing